High-resolution epitope mapping via radical protein footprinting

Learn about a new way to map epitopes – guaranteed success with single-residue resolution in as little as 2 weeks.

Are failure prone x-ray crystallography and cryoEM straining your budget? Get structural verification of your antibody binding sites in just weeks – non-linear epitopes and tough targets included. Our milestoned project approach means each project is de-risked – no more expenses for failed attempts!

You’ll get: 

A concise introduction to modern epitope mapping

A look at the current techniques used to perform epitope mapping

Actionable summaries of protein footprinting for epitope mapping

A case study demonstrating a cutting-edge technique in practice

Considerations for epitope mapping

When you think of small molecule drugs, you think of the binding site, or a “lock-and-key" analogy– similarly, no biologic is complete without fully characterizing and understanding its epitope, or binding site - it’s a must have. Before you settle for a suboptimal approach, give our epitope mapping platform a try. Our technique, epitope mapping by hydroxyl radical protein footprinting (HRPF), circumvents all of the major drawbacks of current approaches — it’s ultra-fast, universally compatible, works in native conditions, and all of our projects are guaranteed.

Traditional techniques?Buyer beware

Common epitope mapping techniques, like x-ray crystallography and cryoEM, are prone to failure, prohibitively expensive, and incompatible with certain classes of targets (e.g. integral membrane proteins and megacomplexes). Other approaches developed to circumvent these issues, like mutagenesis, peptide scanning, and hydrogen-deuterium exchange mass spectrometry, expose the target to non-native conditions, miss non-linear and non-obvious epitopes, and can be unreliable and unreproducible. So what’s a developer to do?

How do we do it?

In this paper, we provide a clear and concise introduction our approach for epitope mapping, explore its essential role in drug development, and explain, with evidence, how we analyze proteins in a fraction of the time and at a fraction of the cost and complexity compared to traditional techniques.


Here’s the main idea.

Mapping the binding sites (or epitopes) of antibodies and their target antigens is critical for understanding their mechanism of action as well as filing patent claims. In addition, understanding the motion and dynamics of the antigen in response to binding provides additional insight to advance therapeutic candidates and maximize efficacy.

Epitope mapping is often relegated to the later stages of discovery because of the challenges with capturing the structural detail of the antibody/antigen interaction. There is one clear cause: the techniques that are commonly used for epitope mapping, including x-ray crystallography, peptide scanning, mutagenesis, and hydrogen-deuterium exchange (HDX), all have significant limitations and drawbacks.

Our orthogonal technique for protein footprinting is called hydroxyl radical protein footprinting - we use our proprietary platform to label proteins and detect changes in solvent accessibility of protein side chains. Top pharmaceutical companies and biotechnology firms are working with Immuto and deploying epitope mapping by HRPF to condense the discovery process and curtail the millions of dollars spent on discovery.

This paper provides ample information and evidence to understand our approach, provides hard data and case studies so you can evaluate its utility, details where it can be applied, and describes how it will give your organization an advantage in streamlining drug discovery and development.

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Everything you need to know about improving your epitope mapping efforts — and novel ways to apply our approach for exponential improvements in your discovery process.

This paper covers the basics of epitope mapping, summarizes the latest published research into its functions and utility, and provides a practical look at how our approach is poised to give a significant competitive advantage at the earliest stages of the development lifecycle.

It's essential reading for anyone involved in therapeutic R&D looking to bring efficiency, depth of insight, and temporal and economic savings to their discovery processes. Ensure that you stay informed of new technologies and techniques for streamlining discovery and development.


See how your development lifecycle stands to gain from the latest techniques in epitope mapping.

Get a practical, applied look at epitope mapping in biologic drug development.

See why epitope mapping is important for antibody discovery.

Explore the current techniques: Hydrogen Deuterium eXchange and Hydroxyl Radical Protein Footprinting

See the benefits of using PLIMB for HRF.


See the technology used by other innovators to streamline biologic development.

We’ve attracted the attention of industry leaders, investors, and clients, including the top pharmaceutical companies and biotechnology firms in the US, and these esteemed institutional partners.

"Unlike other techniques, hydroxyl radical footprinting allows you to see how a protein is moving around in solution. And it’s much faster. It’s extremely useful to any company that wants to engineer the next billion-dollar drug."